ARG22473
anti-EMR1 F4 / 80 antibody [Cl:A3-1] (low endotoxin)
anti-EMR1 F4 / 80 antibody [Cl:A3-1] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Mouse
Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
概述
| 产品描述 | Azide free and low endotoxin Rat Monoclonal antibody [Cl:A3-1] recognizes EMR1 F4 / 80 This antibody recognizes the murine F4 / 80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, FuncSt, IHC-Fr, IHC-P, IP, RIA, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | Cl:A3-1 |
| 同位型 | IgG2b |
| 靶点名称 | EMR1 F4 / 80 |
| 抗原物种 | Mouse |
| 抗原 | Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice. |
| 偶联标记 | Un-conjugated |
| 別名 | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques. FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1 |
|---|---|
| 基因名称 | Adgre1 |
| 全名 | adhesion G protein-coupled receptor E1 |
| 背景介绍 | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
| 产品亮点 | Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
| 预测分子量 | 98 kDa |
