ARG55553

anti-EMR1 F4/80 antibody [BM8]

anti-EMR1 F4/80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse

Controls and Markers antibody; Immune System antibody

概述

产品描述

Rat Monoclonal antibody [BM8] recognizes EMR1 F4/80

反应物种 Hu, Ms
应用 FACS, IHC-Fr, WB
特异性 Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages.
宿主 Rat
克隆 Monoclonal
克隆号 BM8
同位型 IgG2a
靶点名称 EMR1 F4/80
抗原物种 Mouse
抗原 BALB/c macrophages obtained from 14 day old bone marrow cell cultures.
偶联标记 Un-conjugated
全名 adhesion G protein-coupled receptor E1
別名 EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3

应用说明

应用建议
应用 推荐稀释比
FACS1:50
IHC-Fr1:50
WB1:50
应用说明 WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen.
Flow Cytometry: Fix with 1% paraformaldehyde.
IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 IHC-Fr: Spleen
预测分子量 125 kDa

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS, 0.02% Sodium azide and 0.1% BSA
抗菌剂 0.02% Sodium azide
稳定剂 0.1% BSA
浓度 0.1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13733 Mouse ADGRE1

GeneID: 2015 Human ADGRE1

Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1

Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1

基因名称 Adgre1
基因全名 adhesion G protein-coupled receptor E1
背景介绍 This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
生物功能 Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]
研究领域 Controls and Markers antibody; Immune System antibody