ARG55553

anti-EMR1 F4/80 antibody [BM8]

anti-EMR1 F4/80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse

Controls and Markers antibody; Immune System antibody

Overview

Product Description

Rat Monoclonal antibody [BM8] recognizes EMR1 F4/80

Tested Reactivity Hu, Ms
Tested Application FACS, IHC-Fr, WB
Specificity Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages.
Host Rat
Clonality Monoclonal
Clone BM8
Isotype IgG2a
Target Name EMR1 F4/80
Antigen Species Mouse
Immunogen BALB/c macrophages obtained from 14 day old bone marrow cell cultures.
Conjugation Un-conjugated
Full Name adhesion G protein-coupled receptor E1
Alternate Names EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3

Application Instructions

Application Suggestion
Application Dilution
FACS1:50
IHC-Fr1:50
WB1:50
Application Note WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen.
Flow Cytometry: Fix with 1% paraformaldehyde.
IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-Fr: Spleen
Calculated MW 125 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.02% Sodium azide and 0.1% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.1% BSA
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13733 Mouse ADGRE1

GeneID: 2015 Human ADGRE1

Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1

Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1

Gene Symbol Adgre1
Gene Full Name adhesion G protein-coupled receptor E1
Background This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]
Research Area Controls and Markers antibody; Immune System antibody