anti-EMR1 F4/80 antibody [BM8]
anti-EMR1 F4/80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse
Controls and Markers antibody; Immune System antibody
Rat Monoclonal antibody [BM8] recognizes EMR1 F4/80
|Tested Reactivity||Hu, Ms|
|Tested Application||FACS, IHC-Fr, WB|
|Specificity||Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages.|
|Target Name||EMR1 F4/80|
|Immunogen||BALB/c macrophages obtained from 14 day old bone marrow cell cultures.|
|Full Name||adhesion G protein-coupled receptor E1|
|Alternate Names||EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3|
|Application Note||WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen.
Flow Cytometry: Fix with 1% paraformaldehyde.
IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||IHC-Fr: Spleen|
|Calculated MW||125 kDa|
|Purification||Purification with Protein G.|
|Buffer||PBS, 0.02% Sodium azide and 0.1% BSA|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||adhesion G protein-coupled receptor E1|
|Background||This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]|
|Function||Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt]|
|Research Area||Controls and Markers antibody; Immune System antibody|