ARG55553
anti-EMR1 F4 / 80 antibody [BM8]
anti-EMR1 F4 / 80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse
Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
概述
| 产品描述 | Rat Monoclonal antibody [BM8] recognizes EMR1 F4 / 80 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-Fr, WB |
| 特异性 | Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | BM8 |
| 同位型 | IgG2a |
| 靶点名称 | EMR1 F4 / 80 |
| 抗原物种 | Mouse |
| 抗原 | BALB/c macrophages obtained from 14 day old bone marrow cell cultures. |
| 偶联标记 | Un-conjugated |
| 別名 | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3 |
应用说明
| 应用建议 |
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| 应用说明 | WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen. Flow Cytometry: Fix with 1% paraformaldehyde. IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-Fr: Spleen |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.02% Sodium azide and 0.1% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.1% BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1 Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1 |
|---|---|
| 基因名称 | Adgre1 |
| 全名 | adhesion G protein-coupled receptor E1 |
| 背景介绍 | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
| 产品亮点 | Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
| 预测分子量 | 98 kDa |
