ARG67001
anti-Dengue Virus NS1 antibody
anti-Dengue Virus NS1 antibody for ICC/IF,Western blot and Dengue virus
Microbiology and Infectious Disease antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Dengue Virus NS1 |
|---|---|
| 反应物种 | DEN |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dengue virus NS1 |
| 抗原物种 | Virus |
| 抗原 | Recombinant protein corresponding to Dengue virus NS1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | DENV_gp1 |
|---|---|
| 全名 | Dengue virus nonstructural protein 1 |
| 背景介绍 | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| 生物功能 | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| 产品亮点 | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New Dengue Virus NS1 antibodies and Antibody Duo Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response |
| 研究领域 | Microbiology and Infectious Disease antibody |
检测图片 (2) Click the Picture to Zoom In
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ARG67001 anti-Dengue virus NS1 antibody ICC/IF image
Immunofluorescence: DENV2 infected Vero cells or mock infected Vero cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG67001 anti-Dengue virus NS1 antibody .
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ARG67001 anti-Dengue virus NS1 antibody WB image
Western blot: Lysate from BHK-21 cell infected with DENV2 stained with ARG67001 anti-Dengue virus NS1 antibody at 1:1000 dilution, overnight at 4°C.
