ARG57293

anti-Caspase 1 antibody

anti-Caspase 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse

Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
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publication_link 参考文献7

概述

产品描述 Rabbit Polyclonal antibody recognizes Caspase 1
反应物种 Hu, Ms
预测物种 Rat
应用 ICC/IF, IHC-P, IP, WB
特异性 This antibody reacts to full-length and cleaved form of caspase 1 protein.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Caspase 1
抗原物种 Human
抗原 Recombinant Protein of Caspase 1.
偶联标记 Un-conjugated
別名 Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:25 - 1:200
IHC-P1:25 - 1:200
IP1:25 - 1:100
WB1:250 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 1X PBS, 0.025% Sodium azide, 0.025% Thimerosal and 2.5% BSA
抗菌剂 0.025% Sodium azide and 0.025% Thimerosal
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12362 Mouse CASP1

GeneID: 834 Human CASP1

Swiss-port # P29452 Mouse Caspase-1

Swiss-port # P29466 Human Caspase-1

基因名称 CASP1
全名 caspase 1, apoptosis-related cysteine peptidase
背景介绍 Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
生物功能 Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
研究领域 Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
预测分子量 45 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 10 µg of HeLa and 20 µg of Mouse brain lysates stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 25 µg of cell lysate from PMA treated THP-1 cells were stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody ICC/IF image

    Immunofluorescence: Raw264.7 cells stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG57293 anti-Caspase 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG57293 anti-Caspase 1 antibody IP image

    Immunoprecipitation: 200 µg extracts of THP-1 cells Immunoprecipitated and stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

客户反馈

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anti-Caspase 1 antibody

Application:WB

Sample:HeLa and Mouse brain

Sample Loading Amount:10 µg of HeLa and 20 µg of Mouse brain

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Ursolic acid induces apoptosis and pyroptosis in Reh cells by upregulating of the JNK signalling pathway based on network pharmacology and experimental validation

WB / Human

Ying Luo et al.
Heliyon.,  (2023)

publication_link

 

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黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

WB / Rat

王蕾 et al.
中国中西医结合外科杂志,  (2023)

publication_link

 

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Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1

WB / Human

Kai-Che Wei et al.
Microbiol Spectr.,  (2023)

publication_link

 

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Protective effect of ginsenoside Rh2 against Toxoplasma gondii infection-induced neuronal injury through binding TgCDPK1 and NLRP3 to inhibit microglial NLRP3 inflammasome signaling pathway

WB / Mouse

Guang-Nan Jin et al.
Int Immunopharmacol.,  (2022)

publication_link

 

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Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2‑induced pyroptosis

ICC/IF / Human

Yulin Yuan et al.
Mol Med Rep,  (2022)

publication_link

 

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Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2 induced pyroptosis

IF / Huaman

Yulin Yuan et al.
Molecular Medicine Reports,  (2022)

publication_link

 

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Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)

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