ARG11111
anti-Calbindin antibody
anti-Calbindin antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig
概述
| 产品描述 | Chicken Polyclonal antibody recognizes Calbindin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Pig |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | Calbindin |
| 抗原物种 | Human |
| 抗原 | Recombinant full-length Human Calbindin. |
| 偶联标记 | Un-conjugated |
| 別名 | Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 30 or 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS and 5 mM Sodium azide. |
| 抗菌剂 | 5 mM Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CALB1 |
| 全名 | calbindin 1, 28kDa |
| 背景介绍 | The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015] |
| 生物功能 | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt] |
| 预测分子量 | 30 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG11111 anti-Calbindin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11111 anti-Calbindin antibody (green) at 1:2000 dilution, and costained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
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ARG11111 anti-Calbindin antibody WB image
Western blot: Rat cerebellum, Pig hippocampus and Cow cerebellum lysates stained with ARG11111 anti-Calbindin antibody at 1:5000 dilution.
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ARG11111 anti-Calbindin antibody WB image
Western blot: Full length recombinant Human parvalbumin, calretinin and calbindin proteins stained with ARG11111 anti-Calbindin antibody at 1:5000 dilution.
