ARG56421

anti-COX2 antibody

anti-COX2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Inflammation Study antibody
publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes COX2
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 This antibody detects endogenous levels of Cox2 protein.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 COX2
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human Cox2.
偶联标记 Un-conjugated
別名 PHS II; Prostaglandin H2 synthase 2; PHS-2; Cyclooxygenase-2; PGHS-2; COX2; PGG/HS; COX-2; GRIPGHS; hCox-2; PGH synthase 2; Prostaglandin G/H synthase 2; Prostaglandin-endoperoxide synthase 2; EC 1.14.99.1

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
纯度 > 95% (by SDS-PAGE).
缓冲液 PBS (pH 7.2) and 0.05% Sodium azide.
抗菌剂 0.05% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 19225 Mouse PTGS2

GeneID: 29527 Rat PTGS2

GeneID: 5743 Human PTGS2

基因名称 PTGS2
全名 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
背景介绍 COX2: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
生物功能 COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis (PubMed:26859324, PubMed:27226593). Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia. [UniProt]
产品亮点 Related products:
COX2 antibodies; COX2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
研究领域 Inflammation Study antibody
预测分子量 69 kDa
翻译后修饰 S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

检测图片 (2) Click the Picture to Zoom In

  • ARG56421 anti-COX2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG56421 anti-COX2 antibody at 1:100 dilution.

  • ARG56421 anti-COX2 antibody WB image

    Western blot: 1) HeLa whole cell lysate, 2) Jurkat whole cell lysate, 3) Raw264.7 whole cell lysate, and 4) Rat lung lysate stained with ARG56421 anti-COX2 antibody at 1:500 dilution.

文献引用

Antagonistic Activities of Lactobacillus rhamnosus JB3 Against Helicobacter pylori Infection Through Lipid Raft Formation

WB / Human

Anh Duy Do et al.
Front Immunol,  (2022)

publication_link

 

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