ARG64445
anti-CK1 epsilon antibody
anti-CK1 epsilon antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CK1 epsilon |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | Both reported variants represent identical protein (NP_001885.1 and NP_689407.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CK1 epsilon |
| 抗原物种 | Human |
| 抗原 | C-PASQTSVPFDHLGK |
| 偶联标记 | Un-conjugated |
| 別名 | tau; CK1epsilon; Casein kinase I isoform epsilon; AI426939; CKIe; CKI-epsilon; AW457082; AI551861; EC 2.7.11.1; KC1epsilon |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 47 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG64445 anti-CK1 epsilon antibody WB image
Western blot: 1) Mock transfection and 2) CSNK1E expressing plasmid transfected HEK293 cell lysate stained with ARG64445 anti-CK1 epsilon antibody.
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ARG64445 anti-CK1 epsilon antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64445 anti-CK1 epsilon antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64445 anti-CK1 epsilon antibody WB image
Western blot: 35 µg of HEK293 cell lysate (in RIPA buffer) stained with ARG64445 anti-CK1 epsilon antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
