ARG66976
anti-CD62L / L-Selectin antibody
anti-CD62L / L-Selectin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD62L / L-Selectin |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD62L / L-Selectin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to the extracellular domain of CD62L / L-Selectin. |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Leukocyte; Uterus |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SELL |
| 全名 | selectin L |
| 背景介绍 | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells. |
| 生物功能 | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt] |
| 产品亮点 | Related products: CD62L antibodies; CD62L ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 42 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66976 anti-CD62L / L-Selectin antibody ICC/IF image
Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66976 anti-CD62L / L-Selectin antibody (red) at 1:200 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
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ARG66976 anti-CD62L / L-Selectin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tissue stained with ARG66976 anti-CD62L / L-Selectin antibody at 1:100 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
