anti-CD45RB antibody [MEM-55]

anti-CD45RB antibody [MEM-55] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
publication_link 参考文献1



Mouse Monoclonal antibody [MEM-55] recognizes CD45RB

反应物种 Hu, NHuPrm
应用 CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP, WB
特异性 The clone MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
HLDA III; WS Code NL 358
HLDA V; WS Code T T-151
HLDA V; WS Code T T-CD45.08
宿主 Mouse
克隆 Monoclonal
克隆号 MEM-55
同位型 IgG1
靶点名称 CD45RB
抗原物种 Human
抗原 Human thymocytes and T lymphocytes.
偶联标记 Un-conjugated
別名 LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC; B220


应用 推荐稀释比
FACS2 µg/ml
应用说明 IHC-P: No pre-treatment of tissue sections is essential.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purified from ascites by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 5788 Human PTPRC

Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C

基因名称 PTPRC
全名 protein tyrosine phosphatase, receptor type, C
背景介绍 CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
生物功能 CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
产品亮点 Related products:
CD45RB antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
研究领域 Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
预测分子量 147 kDa
翻译后修饰 Heavily N- and O-glycosylated.

检测图片 (2) Click the Picture to Zoom In

  • ARG62865 anti-CD45RB antibody [MEM-55] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG62865 anti-CD45RB antibody [MEM-55].

  • ARG62865 anti-CD45RB antibody [MEM-55] IHC-P image

    Immunohistochemistry: Tonsil (paraffin-embedded sections) stained with ARG62865 anti-CD45RB antibody [MEM-55].


Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.

ICC/IF / Human

Elizabeth Ngoc Hoa Tran et al.
ACS Infect Dis.,  (2020)