ARG65533
anti-CD45R / B220 antibody [RA3-6B2] (low endotoxin)
anti-CD45R / B220 antibody [RA3-6B2] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Mouse,Cat
Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
概述
| 产品描述 | Azide free and low endotoxin Rat Monoclonal antibody [RA3-6B2] recognizes CD45R |
|---|---|
| 反应物种 | Hu, Ms, Cat |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP |
| 特异性 | The clone RA3-6B2 recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | RA3-6B2 |
| 同位型 | IgG2a |
| 靶点名称 | CD45R / B220 |
| 抗原 | Abelson murine leukemia virus-induced pre-B tumor cells |
| 偶联标记 | Un-conjugated |
| 別名 | LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220 |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Modulation of B cell responses. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C |
|---|---|
| 背景介绍 | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| 生物功能 | CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt] |
| 产品亮点 | Related products: CD45R antibodies; CD45R Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody |
| 预测分子量 | 147 kDa |
| 翻译后修饰 | Heavily N- and O-glycosylated. |
