ARG54302
anti-CD3 epsilon antibody [UCHT1] (APC)
anti-CD3 epsilon antibody [UCHT1] (APC) for Flow cytometry and Human,Primates
Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [UCHT1] recognizes CD3 epsilon |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The clone UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex. HLDA I; WS Code T 3 HLDA III; WS Code T 126 HLDA III; WS Code T 471 HLDA VI; WS Code T 6T-CD3.1 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | UCHT1 |
| 同位型 | IgG1 |
| 靶点名称 | CD3 epsilon |
| 抗原物种 | Human |
| 抗原 | human thymocytes followed by Sezary T cells |
| 偶联标记 | APC |
| 別名 | CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain |
|---|---|
| 基因名称 | CD3E |
| 全名 | CD3 Epsilon Subunit Of T-Cell Receptor Complex |
| 背景介绍 | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| 生物功能 | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. |
| 细胞定位 | Cell membrane, Membrane |
| 产品亮点 | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| 预测分子量 | 19 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
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ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) FACS image
Flow Cytometry: Separation of human CD3 epsilon positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
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ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) FACS image
Flow Cytometry: Human Lymphocytes stained with ARG62744 anti-CD16 antibody [LNK16] (FITC) (20 µl reagent / 100 µl of peripheral whole blood) and ARG54302 anti-CD3 epsilon antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
克隆号文献
Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.
Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.
Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.
Identification and characterization of human pulmonary dendritic cells.
Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.
TCR dynamics in human mature T lymphocytes lacking CD3 gamma.
