ARG62801

anti-CD3 epsilon antibody [OKT3] (low endotoxin)

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Functional grade and low endotoxin Mouse Monoclonal antibody [OKT3] recognizes CD3 epsilon
反应物种	Hu
应用	FACS, FuncSt, IHC-Fr
特异性	The clone OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia. HLDA VI; WS Code T6T-R3
宿主	Mouse
克隆	Monoclonal
克隆号	OKT3
同位型	IgG2a
靶点名称	CD3 epsilon
抗原	Commercially sensitive information
偶联标记	Un-conjugated
別名	CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon

应用说明

应用建议

应用	推荐稀释比
FACS	1 µg/ml
FuncSt	Assay-dependent
IHC-Fr	Assay-dependent

应用说明

Functional studies: Counteracting both generation and function of effector T cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
纯化说明	0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 916 Human CD3E Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain
基因名称	CD3E
全名	CD3 Epsilon Subunit Of T-Cell Receptor Complex
背景介绍	The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
生物功能	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
细胞定位	Cell membrane, Membrane
产品亮点	Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response
研究领域	Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
预测分子量	19 kDa

检测图片 (2) Click the Picture to Zoom In

ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) FACS image

Flow Cytometry: Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.