ARG57681

anti-CD274 / PD-L1 antibody [RM320]

anti-CD274 / PD-L1 antibody [RM320] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

publication_link 参考文献4

概述

产品描述 Rabbit Monoclonal antibody [RM320] recognizes CD274 / PD-L1
反应物种 Hu
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 RM320
同位型 IgG
靶点名称 CD274 / PD-L1
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human PD-L1.
偶联标记 Un-conjugated
別名 Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:200 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA.
抗菌剂 0.09% Sodium azide
稳定剂 50% Glycerol and 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 29126 Human CD274

Swiss-port # Q9NZQ7 Human Programmed cell death 1 ligand 1

基因名称 CD274
全名 CD274 molecule
背景介绍 CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能 CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
细胞定位 Cell membrane and Endomembrane system.
产品亮点 Related products:
PD-L1 antibodies; PD-L1 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
The best solution for PD-1/PD-L1 research
Examining CTL/NK-mediated cytotoxicity by ELISA
预测分子量 33 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG57681 anti-CD274 / PD-L1 antibody [RM320] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue section stained with ARG57681 anti-CD274 / PD-L1 antibody [RM320] at 1:1000 dilution.

  • ARG57681 anti-CD274 / PD-L1 antibody [RM320] WB image

    Western blot: H1975, DU145 and LNCaP cell lysates stained with ARG57681 anti-CD274 / PD-L1 antibody [RM320] at 1:1000 dilution.

  • ARG57681 anti-CD274 / PD-L1 antibody [RM320] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung cancer tissue section stained with ARG57681 anti-CD274 / PD-L1 antibody [RM320] at 1:1000 dilution.

文献引用

Molecular and immune characteristics for lung adenocarcinoma patients with CMTM6 overexpression.

IHC-P / Human

Wang Hui et al.
Int Immunopharmacol.,  (2020)

publication_link

 

hr_line

CD44, a marker of cancer stem cells, is positively correlated with PD-L1 expression and immune cells infiltration in lung adenocarcinoma

IHC-P / Human

Chenyue Zhang et al.
Cancer Cell Int.,  (2020)

publication_link

 

hr_line

CMTM6 is positively correlated with PD-L1 expression and immune cells infiltration in lung squamous carcinoma.

IHC-P / Human

Xiaoling Shang et al.
Int Immunopharmacol.,  (2020)

publication_link

 

hr_line

CMTM6 significantly relates to PD-L1 and predicts the prognosis of gastric cancer patients.

IHC-P / Human

Xin Li et al.
PeerJ.,  (2020)

publication_link

 

hr_line