ARG22456
anti-CD206 / MMR antibody [MR5D3]
anti-CD206 / MMR antibody [MR5D3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation and Mouse
Immune System antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
参考文献2
概述
| 产品描述 | Rat Monoclonal antibody [MR5D3] recognizes CD206 / MMR This antibody recognizes the mouse mannose receptor, a 175kD type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells. |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | MR5D3 |
| 同位型 | IgG2a |
| 靶点名称 | CD206 / MMR |
| 抗原物种 | Mouse |
| 抗原 | Chimaeric CRD4-7-Fc protein |
| 偶联标记 | Un-conjugated |
| 別名 | CLEC13D; C-type lectin domain family 13 member D; Macrophage mannose receptor 1-like protein 1; C-type lectin domain family 13 member D-like; MMR; CLEC13DL; CD206; Macrophage mannose receptor 1; bA541I19.1; CD antigen CD206; MRC1L1 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Mrc1 |
| 全名 | mannose receptor, C type 1 |
| 背景介绍 | The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. CD206 / MMR is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. [provided by RefSeq, Sep 2015] |
| 生物功能 | CD206 / MMR mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. (Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E. (Microbial infection) Interacts with Hepatitis B virus envelope protein. [UniProt] |
| 产品亮点 | Related products: CD206 antibodies; CD206 ELISA Kits; CD206 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| 研究领域 | Immune System antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody |
| 预测分子量 | 166 kDa |
检测图片 (7) Click the Picture to Zoom In
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ARG22456 anti-CD206 / MMR antibody [MR5D3] ICC/IF image
Immunofluorescence: Mouse macrophages stained with ARG22456 anti-CD206 / MMR antibody [MR5D3] at 1:200 dilution.
From Qiu-Yue Lin et al. Clin Transl Med. (2021), doi: 10.1002/ctm2.374, Fig. S2.
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ARG22456 anti-CD206 / MMR antibody [MR5D3] FACS image
Flow Cytometry: J774 cell line stained with ARG22456 anti-CD206 / MMR antibody [MR5D3] following permeabilization.
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Low power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Medium power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (High power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (Low power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (High power).
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