ARG22456

anti-CD206 / MMR antibody [MR5D3]

anti-CD206 / MMR antibody [MR5D3] for IHC-Frozen sections,Flow cytometry,ICC/IF,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [MR5D3] recognizes CD206 / MMR
This antibody recognizes the mouse mannose receptor, a 175kD type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells.
Tested Reactivity Ms
Tested Application FACS, ICC/IF, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone MR5D3
Isotype IgG2a
Target Name CD206 / MMR
Antigen Species Mouse
Immunogen Chimaeric CRD4-7-Fc protein
Conjugation Un-conjugated
Full Name mannose receptor, C type 1
Alternate Names CLEC13D; C-type lectin domain family 13 member D; Macrophage mannose receptor 1-like protein 1; C-type lectin domain family 13 member D-like; MMR; CLEC13DL; CD206; Macrophage mannose receptor 1; bA541I19.1; CD antigen CD206; MRC1L1

Application Instructions

Application Suggestion
Application Dilution
FACS1:10 - 1:20
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17533 Mouse MRC1

Swiss-port # Q61830 Mouse Macrophage mannose receptor 1

Gene Symbol Mrc1
Gene Full Name mannose receptor, C type 1
Background The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]
Function Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. [UniProt]

Images (6) Click the Picture to Zoom In

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] FACS image

    Flow Cytometry: J774 cell line stained with ARG22456 anti-CD206 / MMR antibody [MR5D3] following permeabilization.

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Low power).

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Medium power).

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (High power).

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (Low power).

  • ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image

    Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (High power).