ARG22456
anti-CD206 / MMR antibody [MR5D3]
anti-CD206 / MMR antibody [MR5D3] for IHC-Frozen sections,Flow cytometry,ICC/IF,Immunoprecipitation and Mouse
Overview
| Product Description | Rat Monoclonal antibody [MR5D3] recognizes CD206 / MMR This antibody recognizes the mouse mannose receptor, a 175kD type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IP |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | MR5D3 |
| Isotype | IgG2a |
| Target Name | CD206 / MMR |
| Antigen Species | Mouse |
| Immunogen | Chimaeric CRD4-7-Fc protein |
| Conjugation | Un-conjugated |
| Full Name | mannose receptor, C type 1 |
| Alternate Names | CLEC13D; C-type lectin domain family 13 member D; Macrophage mannose receptor 1-like protein 1; C-type lectin domain family 13 member D-like; MMR; CLEC13DL; CD206; Macrophage mannose receptor 1; bA541I19.1; CD antigen CD206; MRC1L1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database links | |
|---|---|
| Gene Symbol | Mrc1 |
| Gene Full Name | mannose receptor, C type 1 |
| Background | The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015] |
| Function | Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG22456 anti-CD206 / MMR antibody [MR5D3] FACS image
Flow Cytometry: J774 cell line stained with ARG22456 anti-CD206 / MMR antibody [MR5D3] following permeabilization.
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Low power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (Medium power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3]. (High power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (Low power).
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ARG22456 anti-CD206 / MMR antibody [MR5D3] IHC-Fr image
Immunohistochemistry: Mouse lymph node cryosection stained with ARG22456 anti-CD206 / MMR antibody [MR5D3], green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI. (High power).
