ARG45400
anti-CD148 / DEP1 antibody
anti-CD148 / DEP1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD148 / DEP1 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD148 / DEP1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human CD148 / DEP1. |
| 偶联标记 | Un-conjugated |
| 別名 | R-PTP-J; Density-enhanced phosphatase 1; R-PTP-ETA; DEP-1; SCC1; DEP1; CD antigen CD148; Receptor-type tyrosine-protein phosphatase eta; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; EC 3.1.3.48; HPTPeta; HPTP eta; R-PTP-eta; CD148 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 240 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12913 Human Receptor-type tyrosine-protein phosphatase eta |
|---|---|
| 基因名称 | PTPRJ |
| 全名 | protein tyrosine phosphatase, receptor type, J |
| 背景介绍 | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. [UniProt] |
| 细胞定位 | Cell junction; Cell membrane; Cell projection; Membrane; Secreted. [UniProt] |
| 预测分子量 | 146 kDa |
| 翻译后修饰 | N- and O-glycosylated. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45400 anti-CD148 / DEP1 antibody IHC-P image
Immunohistochemistry: Human rectal cancer stained with ARG45400 anti-CD148 / DEP1 antibody at 2 μg/ml dilution.
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ARG45400 anti-CD148 / DEP1 antibody ICC/IF image
Immunofluorescence: Human liver cancer stained with ARG45400 anti-CD148 / DEP1 antibody at 5 μg/ml dilution.
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ARG45400 anti-CD148 / DEP1 antibody WB image
Western blot: A549 stained with ARG45400 anti-CD148 / DEP1 antibody at 0.5 μg/ml dilution.
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ARG45400 anti-CD148 / DEP1 antibody FACS image
Flow Cytometry: HEL stained with ARG45400 anti-CD148 / DEP1 antibody at 1 µg/10^6 cells dilution.
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ARG45400 anti-CD148 / DEP1 antibody WB image
Western blot: Rat brain stained with ARG45400 anti-CD148 / DEP1 antibody at 0.5 μg/ml dilution.
