ARG22000

anti-CD11b antibody [M1/70]

anti-CD11b antibody [M1/70] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation,Blocking and Mouse,Human,Rhesus Monkey,Baboon

Overview

Product Description Rat Monoclonal antibody [M1/70] recognizes CD11b
Tested Reactivity Hu, Ms, Bb, R. Mk
Tested Application BL, FACS, ICC/IF, IHC-Fr, IHC-P, IP
Specificity Mouse/Human/Rhesus/Baboon CD11b
Host Rat
Clonality Monoclonal
Clone M1/70
Isotype IgG2bκ
Target Name CD11b
Antigen Species Mouse
Immunogen C57BL/10 Mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
Conjugation Un-conjugated
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16409 Mouse ITGAM

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt]