ARG65478

anti-CD11b antibody [M1/70] (APC)

anti-CD11b antibody [M1/70] (APC) for Flow cytometry and Human,Mouse,Primates,Rabbit

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description

APC-conjugated Rat Monoclonal antibody [M1/70] recognizes CD11b

Tested Reactivity Hu, Ms, NHuPrm, Rb
Tested Application FACS
Specificity The clone M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Host Rat
Clonality Monoclonal
Clone M1/70
Isotype Rat IgG2b
Target Name CD11b
Antigen Species Mouse
Immunogen B10 mouse spleen cells enriched for T cells
Conjugation APC
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Application Dilution
FACSAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16409 Mouse ITGAM

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Clone References

Experimental autoimmune prostatitis induces microglial activation in the spinal cord.

Wong L et al.
Prostate.,  (2015)

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hr_line

Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells.

WB / Mouse

Dang Y et al.
PLoS One.,  (2014)

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hr_line

VEGFR-1 blockade disrupts peri-implantation decidual angiogenesis and macrophage recruitment.

IHC / Mouse

Douglas NC et al.
Vasc Cell.,  (2014)

publication_link

 

hr_line

Astrocyte-derived BDNF supports myelin protein synthesis after cuprizone-induced demyelination.

IHC-FoFr / Mouse

Fulmer CG et al.
J Neurosci.,  (2014)

publication_link

 

hr_line

Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.

Thorne AH et al.
Mol Ther.,  (2014)

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hr_line

Retinoid-X-receptors (α/β) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.

ICC/IF / Mouse

Coleman DJ et al.
PLoS Genet.,  (2014)

publication_link

 

hr_line

Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.

IHC-Fr / Mouse

Liu T et al.
Sci Rep.,  (2014)

publication_link

 

hr_line

Intrastriatal transplantation of adenovirus-generated induced pluripotent stem cells for treating neuropathological and functional deficits in a rodent model of Huntington's disease.

Fink KD et al.
Stem Cells Transl Med.,  (2014)

publication_link

 

hr_line

Neurons are host cells for Mycobacterium tuberculosis.

Randall PJ et al.
Infect Immun.,  (2014)

publication_link

 

hr_line

MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS.

IHC-FoFr / Mouse

Kawaguchi-Niida M et al.
Acta Neuropathol Commun.,  (2013)

publication_link

 

hr_line

Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

FACS / Mouse

Olme CH et al.
Leuk Res.,  (2013)

publication_link

 

hr_line

Matricellular protein CCN1 activates a proinflammatory genetic program in murine macrophages.

FuncSt / Mouse

Bai T et al.
J Immunol.,  (2010)

publication_link

 

hr_line

Surfactant protein A modulates cell surface expression of CR3 on alveolar macrophages and enhances CR3-mediated phagocytosis.

FACS / 

Gil M et al.
J Biol Chem.,  (2009)

publication_link

 

hr_line

Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.

IHC-Fr / Rabbit

Tamagawa E et al.
Am J Physiol Lung Cell Mol Physiol.,  (2008)

publication_link

 

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