ARG65463

anti-CD11b antibody [ICRF44]

anti-CD11b antibody [ICRF44] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Functional study and Human,Primates

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

概述

产品描述

Mouse Monoclonal antibody [ICRF44] recognizes CD11b

反应物种 Hu, NHuPrm
应用 FACS, FuncSt, ICC/IF, IHC-Fr, IP
特异性 The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
宿主 Mouse
克隆 Monoclonal
克隆号 ICRF44
同位型 IgG1
靶点名称 CD11b
抗原物种 Human
抗原 Rheumatoid synovial cells and human monocytes.
偶联标记 Un-conjugated
全名 integrin, alpha M (complement component 3 receptor 3 subunit)
別名 MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

应用说明

应用说明
Flow Cytometry:
Immunoprecipitation:
Immunohistochemistry (frozen sections):
Immunocytochemistry:
Functional Application: blocking of granulocyte activation
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from cell culture supernatant by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

基因名称 ITGAM
基因全名 integrin, alpha M (complement component 3 receptor 3 subunit)
背景介绍 CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
生物功能 Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt]
研究领域 Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

克隆号文献

Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.

IHC-Fr / Human

Zenkel M et al.
Am J Pathol.,  (2010)

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hr_line

Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.

BL / Human

Harler MB et al.
J Immunol.,  (1999)

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