ARG65463

anti-CD11b antibody [ICRF44]

anti-CD11b antibody [ICRF44] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Functional study and Human,Primates

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [ICRF44] recognizes CD11b

Tested Reactivity Hu, NHuPrm
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
Host Mouse
Clonality Monoclonal
Clone ICRF44
Isotype IgG1
Target Name CD11b
Antigen Species Human
Immunogen Rheumatoid synovial cells and human monocytes.
Conjugation Un-conjugated
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Note
Flow Cytometry:
Immunoprecipitation:
Immunohistochemistry (frozen sections):
Immunocytochemistry:
Functional Application: blocking of granulocyte activation
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Function Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt]
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Clone References

Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.

IHC-Fr / Human

Zenkel M et al.
Am J Pathol.,  (2010)

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Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.

BL / Human

Harler MB et al.
J Immunol.,  (1999)

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