ARG65463
anti-CD11b antibody [ICRF44]
anti-CD11b antibody [ICRF44] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,CyTOF®-candidate and Human,Primates
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [ICRF44] recognizes CD11b |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| 特异性 | The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. HLDA IV, WS Code M047 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ICRF44 |
| 同位型 | IgG1 |
| 靶点名称 | CD11b |
| 抗原物种 | Human |
| 抗原 | Rheumatoid synovial cells and human monocytes. |
| 偶联标记 | Un-conjugated |
| 全名 | integrin, alpha M (complement component 3 receptor 3 subunit) |
| 別名 | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Blocking of granulocyte activation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGAM |
| 基因全名 | integrin, alpha M (complement component 3 receptor 3 subunit) |
| 背景介绍 | CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
| 生物功能 | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt] |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
克隆号文献
Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.
IHC-Fr / Human
Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.
BL / Human
