anti-CD11b antibody [ICRF44]
anti-CD11b antibody [ICRF44] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,CyTOF®-candidate and Human,Primates
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Mouse Monoclonal antibody [ICRF44] recognizes CD11b
|应用||CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP|
|特异性||The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
|抗原||Rheumatoid synovial cells and human monocytes.|
|全名||integrin, alpha M (complement component 3 receptor 3 subunit)|
|別名||MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B|
|应用说明||Functional studies: Blocking of granulocyte activation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purified from cell culture supernatant by protein-A affinity chromatography.|
|纯度||> 95% (by SDS-PAGE)|
|缓冲液||PBS (pH 7.4) and 15 mM Sodium azide|
|抗菌剂||15 mM Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|基因全名||integrin, alpha M (complement component 3 receptor 3 subunit)|
|背景介绍||CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.|
|生物功能||Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt]|
|研究领域||Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody|