ARG65463
anti-CD11b antibody [ICRF44]
anti-CD11b antibody [ICRF44] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Functional study and Human,Primates
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [ICRF44] recognizes CD11b |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| Specificity | The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. HLDA IV, WS Code M047 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ICRF44 |
| Isotype | IgG1 |
| Target Name | CD11b |
| Antigen Species | Human |
| Immunogen | Rheumatoid synovial cells and human monocytes. |
| Conjugation | Un-conjugated |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Alternate Names | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
Application Instructions
| Application Note | Flow Cytometry: Immunoprecipitation: Immunohistochemistry (frozen sections): Immunocytochemistry: Functional Application: blocking of granulocyte activation * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database links | |
|---|---|
| Gene Symbol | ITGAM |
| Gene Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Background | CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
| Function | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. [UniProt] |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
Clone References
Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.
IHC-Fr / Human
Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.
BL / Human
