anti-CD11b antibody [ICRF44]
anti-CD11b antibody [ICRF44] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,CyTOF®-candidate and Human,Primates
MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Mouse Monoclonal antibody [ICRF44] recognizes CD11b
|应用||CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP|
|特异性||The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
|抗原||Rheumatoid synovial cells and human monocytes.|
|全名||integrin, alpha M (complement component 3 receptor 3 subunit)|
|別名||MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B|
|应用说明||Functional studies: Blocking of granulocyte activation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purified from cell culture supernatant by protein-A affinity chromatography.|
|纯度||> 95% (by SDS-PAGE)|
|缓冲液||PBS (pH 7.4) and 15 mM Sodium azide|
|抗菌剂||15 mM Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|基因全名||integrin, alpha M (complement component 3 receptor 3 subunit)|
|背景介绍||CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]|
|生物功能||CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]|
CD11b antibodies; CD11b Duos / Panels; Anti-Mouse IgG secondary antibodies;
New antibody panels and duos for Tumor immune microenvironment
|研究领域||MDSC Marker antibody; Myeloid-derived suppressor cell antibody|