ARG65463

anti-CD11b antibody [ICRF44]

anti-CD11b antibody [ICRF44] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,CyTOF®-candidate and Human,Primates

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

概述

产品描述

Mouse Monoclonal antibody [ICRF44] recognizes CD11b

反应物种 Hu, NHuPrm
应用 CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
特异性 The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
宿主 Mouse
克隆 Monoclonal
克隆号 ICRF44
同位型 IgG1
靶点名称 CD11b
抗原物种 Human
抗原 Rheumatoid synovial cells and human monocytes.
偶联标记 Un-conjugated
全名 integrin, alpha M (complement component 3 receptor 3 subunit)
別名 MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

应用说明

应用建议
应用 推荐稀释比
CyTOF®-candidateAssay-dependent
FACSAssay-dependent
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
应用说明 Functional studies: Blocking of granulocyte activation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 127 kDa

属性

形式 Liquid
纯化 Purified from cell culture supernatant by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

基因名称 ITGAM
基因全名 integrin, alpha M (complement component 3 receptor 3 subunit)
背景介绍 CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
生物功能 CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
产品亮点 Related products:
CD11b antibodies; CD11b Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
研究领域 MDSC Marker antibody; Myeloid-derived suppressor cell antibody

克隆号文献

Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.

IHC-Fr / Human

Zenkel M et al.
Am J Pathol.,  (2010)

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Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.

BL / Human

Harler MB et al.
J Immunol.,  (1999)

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