ARG65463

anti-CD11b antibody [ICRF44]

Datasheet

订购信息

包装

价格 (USD)

100 μg

389

arigo in CHINA:
恩瑞科生物
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0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [ICRF44] recognizes CD11b
反应物种	Hu, NHuPrm
应用	CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
特异性	The clone ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. HLDA IV, WS Code M047
宿主	Mouse
克隆	Monoclonal
克隆号	ICRF44
同位型	IgG1
靶点名称	CD11b
抗原物种	Human
抗原	Rheumatoid synovial cells and human monocytes.
偶联标记	Un-conjugated
別名	MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

应用说明

应用建议

应用	推荐稀释比
CyTOF®-candidate	Assay-dependent
FACS	1 - 4 µg/ml
FuncSt	Assay-dependent
ICC/IF	Assay-dependent
IHC-Fr	Assay-dependent
IP	Assay-dependent

应用说明

Functional studies: Blocking of granulocyte activation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from cell culture supernatant by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3684 Human ITGAM Swiss-port # P11215 Human Integrin alpha-M
基因名称	ITGAM
全名	integrin, alpha M (complement component 3 receptor 3 subunit)
背景介绍	CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
生物功能	CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
产品亮点	Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy
研究领域	MDSC Marker antibody; Myeloid-derived suppressor cell antibody
预测分子量	127 kDa

检测图片 (2) Click the Picture to Zoom In

ARG65463 anti-CD11b antibody [ICRF44] FACS image

Flow Cytometry: Human peripheral blood stained with ARG65463 anti-CD11b antibody [ICRF44] at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65463 anti-CD11b antibody [ICRF44] FACS image

Flow Cytometry: Separation of human neutrophil granulocytes (red-filled) from CD11b negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65463 anti-CD11b antibody [ICRF44] at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.