ARG22265
anti-CD107b / LAMP2 antibody [GL2A7]
anti-CD107b / LAMP2 antibody [GL2A7] for Flow cytometry,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rabbit
概述
| 产品描述 | Rat Monoclonal antibody [GL2A7] recognizes CD107b / LAMP2 |
|---|---|
| 反应物种 | Hu, Ms, Rb |
| 应用 | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, IP, WB |
| 特异性 | Detects ~100-110kDa. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | GL2A7 |
| 同位型 | IgG1 |
| 靶点名称 | CD107b / LAMP2 |
| 抗原物种 | Mouse |
| 抗原 | Purified preparation of Mouse liver lysosomal membranes |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
应用说明
| 应用建议 |
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | For best ICC/IF results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||||
| 实际分子量 | ~ 100-110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 Swiss-port # P17047 Mouse Lysosome-associated membrane glycoprotein 2 |
|---|---|
| 基因名称 | Lamp2 |
| 全名 | lysosomal-associated membrane protein 2 |
| 背景介绍 | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| 细胞定位 | Cell membrane, Endosome, Endosome membrane, Lysosome, Lysosome membrane |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
检测图片 (1) Click the Picture to Zoom In
-
ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] WB image
Western blot: 1) MW ladder, 2) 20 µg of HEK293, and 3) 10 µg of NIH3T3 lysates stained with ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] at 1:500 dilution (60 min at RT). Block: 5% milk + TBST 1hr at RT.
