ARG53997
anti-CBX3 / HP1 gamma antibody
anti-CBX3 / HP1 gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Hamster,Monkey
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CBX3 / HP1 gamma |
|---|---|
| 反应物种 | Hu, Ms, Rat, Hm, Mk |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | CBX3 / HP1 gamma |
| 抗原物种 | Human |
| 抗原 | Purified recombinant Human HP1 gamma protein fragments. |
| 偶联标记 | Un-conjugated |
| 別名 | HP1-GAMMA; Chromobox protein homolog 3; HP1Hs-gamma; HP1 gamma; HECH; Heterochromatin protein 1 homolog gamma; Modifier 2 protein |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 22 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 0.75 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CBX3 |
| 全名 | chromobox homolog 3 |
| 背景介绍 | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| 生物功能 | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. [UniProt] |
| 细胞定位 | Nucleus |
| 产品亮点 | Related Antibody Duos and Panels: ARG30341 Heterochromatin Foci Antibody Duo Related products: CBX3 antibodies; CBX3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
检测图片 (4) Click the Picture to Zoom In
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ARG53997 anti-CBX3 / HP1 gamma antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:200 dilution.
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ARG53997 anti-CBX3 / HP1 gamma antibody IHC-P image
Immunohistochemistry: Paraffin-embedded prostate cancer tissue stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG53997 anti-CBX3 / HP1 gamma antibody WB image
Western blot: 3T3 cell lysate stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:1000 dilution.
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ARG53997 anti-CBX3 / HP1 gamma antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG53997 anti-CBX3 / HP1 gamma antibody.
