ARG55885

anti-Bmi 1 antibody

anti-Bmi 1 antibody for ICC/IF,Western blot and Human,Mouse

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publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes Bmi 1
反应物种 Hu, Ms
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Bmi 1
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 281-314 (C-terminus) of Human Bmi 1.
偶联标记 Un-conjugated
別名 Polycomb complex protein BMI-1; RING finger protein 51; Polycomb group RING finger protein 4

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:25
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 COMMD3-BMI1
全名 COMMD3-BMI1 readthrough
背景介绍 This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]
生物功能 Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. [UniProt]
细胞定位 Nucleus. Cytoplasm.
预测分子量 37 kDa
翻译后修饰 Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

检测图片 (2) Click the Picture to Zoom In

  • ARG55885 anti-Bmi 1 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55885 anti-Bmi 1 antibody at 1:1000 dilution.

  • ARG55885 anti-Bmi 1 antibody ICC/IF image

    Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG55885 anti-Bmi 1 antibody at 1:25 dilution (green). Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (red) at 1:100 dilution.

客户反馈

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anti-Bmi 1 antibody

Application:WB

Sample:HeLa and A549

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

WB / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)

publication_link

 

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