ARG55885
anti-Bmi 1 antibody
anti-Bmi 1 antibody for ICC/IF,Western blot and Human,Mouse

1

概述
产品描述 | Rabbit Polyclonal antibody recognizes Bmi 1 |
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反应物种 | Hu, Ms |
应用 | ICC/IF, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Bmi 1 |
抗原物种 | Human |
抗原 | KLH-conjugated synthetic peptide corresponding to aa. 281-314 (C-terminus) of Human Bmi 1. |
偶联标记 | Un-conjugated |
別名 | Polycomb complex protein BMI-1; RING finger protein 51; Polycomb group RING finger protein 4 |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
基因名称 | COMMD3-BMI1 |
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全名 | COMMD3-BMI1 readthrough |
背景介绍 | This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011] |
生物功能 | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. [UniProt] |
细胞定位 | Nucleus. Cytoplasm. |
预测分子量 | 37 kDa |
翻译后修饰 | Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG55885 anti-Bmi 1 antibody WB image
Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55885 anti-Bmi 1 antibody at 1:1000 dilution.
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ARG55885 anti-Bmi 1 antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG55885 anti-Bmi 1 antibody at 1:25 dilution (green). Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (red) at 1:100 dilution.
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