ARG62367
anti-Bak antibody
anti-Bak antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Hamster
Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Bak |
|---|---|
| 反应物种 | Hu, Ms, Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Bak |
| 抗原物种 | Human |
| 抗原 | A synthetic peptide from the N terminus of human Bak protein. |
| 抗原表位 | N-terminal |
| 偶联标记 | Un-conjugated |
| 別名 | Apoptosis regulator BAK; BCL2L7; Bcl-2-like protein 7; CDN1; Bcl2-L-7; Bcl-2 homologous antagonist/killer; BAK-LIKE; BAK |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A431 cells. Tonsil. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified Antibody |
| 缓冲液 | 1X PBS and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O08734 Mouse Bcl-2 homologous antagonist/killer Swiss-port # Q16611 Human Bcl-2 homologous antagonist/killer |
|---|---|
| 基因名称 | BAK1 |
| 全名 | BCL2-antagonist/killer 1 |
| 背景介绍 | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| 生物功能 | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30275 Pro-apoptotic Bcl2 protein Antibody Panel (BAX, BAK, Bid) Related products: Bak antibodies; Bak Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Pro-apoptotic Bcl2 protein antibody |
| 预测分子量 | 23 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62367 anti-Bak antibody WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG62367 anti-Bak antibody at 1:500 dilution.
客户反馈
Good
anti-Bak antibody
Application：WB
Sample：Mouse lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Bak antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
