ARG57215

anti-B Raf (V600E) antibody [RM8]

Datasheet

订购信息

包装

价格 (USD)

50 μg

489

arigo in CHINA:
恩瑞科生物
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0571-8708-1890

概述

产品描述	Rabbit Monoclonal antibody [RM8] recognizes B Raf (V600E)
反应物种	Hu
应用	ELISA, FACS, ICC/IF, IHC-P, WB
特异性	This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
宿主	Rabbit
克隆	Monoclonal
克隆号	RM8
同位型	IgG
靶点名称	B Raf (V600E)
抗原物种	Human
抗原	Synthetic peptide from Human BRAF V600E mutant.
偶联标记	Un-conjugated
別名	BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA.
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol and 1% BSA
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 673 Human BRAF Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf
基因名称	BRAF
全名	B-Raf proto-oncogene, serine/threonine kinase
背景介绍	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
生物功能	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
预测分子量	84 kDa
翻译后修饰	Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

检测图片 (5) Click the Picture to Zoom In

ARG57215 anti-B Raf (V600E) antibody [RM8] ICC/IF image

Immunocytochemistry: Human WiDr cells stained with ARG57215 anti-B Raf (V600E) antibody [RM8]. (400X, Positive).
ARG57215 anti-B Raf (V600E) antibody [RM8] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded melanoma tissue sections stained with ARG57215 anti-B Raf (V600E) antibody [RM8].
ARG57215 anti-B Raf (V600E) antibody [RM8] WB image

Western blot: Cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein. The blots were stained with ARG57215 anti-B Raf (V600E) antibody [RM8].
ARG57215 anti-B Raf (V600E) antibody [RM8] ELISA image

Sandwich ELISA: Titration curve of ARG57215 anti-B Raf (V600E) antibody [RM8]. Antigen: Lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins.

His-tag fusion proteins were captured by an anti-His-Tag antibody.
ARG57215 anti-B Raf (V600E) antibody [RM8] ICC/IF image

Immunocytochemistry: Human SW40 cells stained with ARG57215 anti-B Raf (V600E) antibody [RM8]. (400X, Negative).