ARG59026
anti-Annexin A2 antibody
anti-Annexin A2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Annexin A2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Annexin A2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 121-141 of Human Annexin A2 (RAEDGSVIDYELIDQDARDLY). |
| 偶联标记 | Un-conjugated |
| 別名 | Placental anticoagulant protein IV; LPC2; Protein I; Calpactin-1 heavy chain; Calpactin I heavy chain; p36; CAL1H; ANX2; LPC2D; Lipocortin II; ANX2L4; HEL-S-270; LIP2; Annexin II; Annexin A2; Annexin-2; P36; Chromobindin-8; PAP-IV |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANXA2 |
| 全名 | annexin A2 |
| 背景介绍 | This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. [UniProt] |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG59026 anti-Annexin A2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG59026 anti-Annexin A2 antibody.
-
ARG59026 anti-Annexin A2 antibody WB image
Western blot: Rat testis, Rat lung, Rat ovary, MCF-7, SMMC-7721, A549 and Jurkat lysates stained with ARG59026 anti-Annexin A2 antibody.
-
ARG59026 anti-Annexin A2 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Mouse spleen and NIH/3T3 whole cell lysates stained with ARG59026 anti-Annexin A2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
