ARG10694

anti-Aldolase C antibody [1A1]

anti-Aldolase C antibody [1A1] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

概述

产品描述 Mouse Monoclonal antibody [1A1] recognizes Aldolase C
反应物种 Hu, Ms, Rat, Cow, Hrs, Pig
预测物种 Chk
应用 ICC/IF, IHC-Fr, WB
宿主 Mouse
克隆 Monoclonal
克隆号 1A1
同位型 IgG1
靶点名称 Aldolase C
抗原 C-terminal sequence KYEGSGEDGGAAAQSLYIANHAY
偶联标记 Un-conjugated
別名 EC 4.1.2.13; Brain-type aldolase; Fructose-bisphosphate aldolase C; ALDC

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification.
缓冲液 PBS and 50% Glycerol.
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11676 Mouse ALDOC

GeneID: 230 Human ALDOC

GeneID: 24191 Rat ALDOC

基因名称 ALDOC
全名 aldolase C, fructose-bisphosphate
背景介绍 This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]
预测分子量 39 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG10694 anti-Aldolase C antibody [1A1] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10694 anti-Aldolase C antibody [1A1] (green) and co-stained with rabbit antibody to NeuN/FOX3 antibody (RPCA-FOX3, red). MCA-1A1 antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit Fox3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.

  • ARG10694 anti-Aldolase C antibody [1A1] WB image

    Western blot: Crude Rat brain lysates stained with ARG10694 anti-Aldolase C antibody [1A1]. Other studies show that this antibody is completely specific for Aldolase C, and does not react with recombinant Human Aldolase A or B.

  • ARG10694 anti-Aldolase C antibody [1A1] WB image

    Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10694 anti-Aldolase C antibody [1A1] is within the C-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 4A9 binds to the N-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.