ARG55168
anti-ATG13 antibody
anti-ATG13 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Death antibody; Metabolism antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG13 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG13 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human ATG13. |
| 偶联标记 | Un-conjugated |
| 別名 | KIAA0652; Autophagy-related protein 13; PARATARG8 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | CEM |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG13 |
| 全名 | autophagy related 13 |
| 生物功能 | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. |
| 研究领域 | Cancer antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
检测图片 (2) Click the Picture to Zoom In
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ARG55168 anti-ATG13 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55168 anti-ATG13 antibody.
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ARG55168 anti-ATG13 antibody WB image
Western blot: 35 µg of CEM cell lysate stained with ARG55168 anti-ATG13 antibody.
