概述

产品描述	Rabbit Polyclonal antibody recognizes ATG12
反应物种	Hu
应用	ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ATG12
抗原物种	Human
抗原	Synthetic peptide derived from Human ATG12.
偶联标记	Un-conjugated
別名	Ubiquitin-like protein ATG12; FBR93; HAPG12; APG12-like; Autophagy-related protein 12; APG12L; APG12

应用说明

应用建议	应用 推荐稀释比 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	293
实际分子量	~ 18 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 9140 Human ATG12 Swiss-port # O94817 Human Ubiquitin-like protein ATG12
基因名称	ATG12
全名	autophagy related 12
背景介绍	Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
生物功能	Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. [UniProt]
细胞定位	Preautophagosomal structure membrane > Peripheral membrane protein. [UniProt]
预测分子量	15 kDa
翻译后修饰	Acetylated by EP300. [UniProt]

检测图片 (1) Click the Picture to Zoom In