ARG66211

anti-ASC / TMS1 antibody

anti-ASC / TMS1 antibody for Western blot and Human,Mouse

NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
publication_link 参考文献1

概述

产品描述

Rabbit Polyclonal antibody recognizes ASC / TMS1

反应物种 Hu, Ms
预测物种 Rat
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 ASC / TMS1
抗原物种 Human
抗原 Fusion protein of Human ASC / TMS1.
偶联标记 Un-conjugated
別名 Apoptosis-associated speck-like protein containing a CARD; TMS; PYD and CARD domain-containing protein; hASC; ASC; TMS-1; TMS1; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1; CARD5

应用说明

应用建议
应用 推荐稀释比
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HL60, A549, Raji and RAW264.7 cells.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol.
抗菌剂 0.05% Sodium azide
稳定剂 40% Glycerol
浓度 1.28 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 29108 Human PYCARD

GeneID: 66824 Mouse PYCARD

Swiss-port # Q9EPB4 Mouse Apoptosis-associated speck-like protein containing a CARD

Swiss-port # Q9ULZ3 Human Apoptosis-associated speck-like protein containing a CARD

基因名称 PYCARD
全名 PYD and CARD domain containing
背景介绍 ASC / TMS1 is an adaptor protein. It is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能 ASC / TMS1 functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
TMS1 antibodies; TMS1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
研究领域 NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
预测分子量 22 kDa
翻译后修饰 Phosphorylated.

检测图片 (1) Click the Picture to Zoom In

  • ARG66211 anti-ASC / TMS1 antibody WB image

    Western blot: 40 µg of HL60, A549, Raji and RAW264.7 cell lysates stained with ARG66211 anti-ASC / TMS1 antibody at 1:500 dilution.

文献引用

黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

WB / Rat

王蕾 et al.
中国中西医结合外科杂志,  (2023)

publication_link

 

hr_line