ARG43689
anti-APC4 antibody
anti-APC4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes APC4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | APC4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to Human APC4. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Anaphase-promoting complex subunit 4 |
| 別名 | Anaphase-promoting complex subunit 4; Cyclosome subunit 4; APC4 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 | ||||||
| 实际分子量 | 95-100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.05% Sodium azide and 40% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UJX5 Human Anaphase-promoting complex subunit 4 |
|---|---|
| 基因名称 | ANAPC4 |
| 全名 | anaphase promoting complex subunit 4 |
| 背景介绍 | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 细胞定位 | Nucleus [UniProt] |
| 预测分子量 | 92 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation |
检测图片 (2) Click the Picture to Zoom In
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ARG43689 anti-APC4 antibody WB image
Western blot: HeLa, 231 and HepG2 cells lysates stained with ARG43689 anti-APC4 antibody at 1:1000 dilution.
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ARG43689 anti-APC4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain stained with ARG43689 anti-APC4 antibody at 1:75 dilution. Antigen Retrieval: Heat mediated was performed using sodium Citrate buffer (pH 6.0).
