ARG63121

anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1]

anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Other

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
publication_link 参考文献2

概述

产品描述

Mouse Monoclonal antibody [MoBu-1] recognizes 5-bromodeoxyuridine / BrdU

反应物种 Other
应用 FACS, ICC/IF, IHC-Fr, IHC-P
特异性 The clone MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). The clone does not cross-react to EdU (PMID: 23272138).
宿主 Mouse
克隆 Monoclonal
克隆号 MoBu-1
同位型 IgG1
靶点名称 5-bromodeoxyuridine / BrdU
抗原 5-bromodeoxyuridine conjugated with hemocyanine.
偶联标记 Un-conjugated

应用说明

应用建议
应用 推荐稀释比
FACS1 - 2 µg/ml
ICC/IF2 µg/ml
IHC-FrAssay-dependent
IHC-PAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from ascites by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍 Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
产品亮点 Related products:
BrdU antibodies; BrdU Duos / Panels; Anti-Mouse IgG secondary antibodies;
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研究领域 Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody

检测图片 (2) Click the Picture to Zoom In

  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] FACS image

    Flow Cytometry: 5-bromodeoxyuridin (BrdU) incorporated CEM cells stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1], followed by incubation with FITC-labelled secondary antibody.

  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] IHC-P image

    Immunohistochemistry: Bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1].

文献引用

Vascular Endothelial Cell-derived Exosomes Protect Neural Stem Cells Against Ischemia/reperfusion Injury.

IHC-Fr / Other

Zhou Shaoting et al.
Neuroscience.,  (2020)

publication_link

 

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Activation of AMPK pathway compromises Rab11 downregulation-mediated inhibition of Schwann cell proliferation in a Glut1 and Glut3-dependent manner.

ICC/IF / Other

Li Fan et al.
Neurosci Lett.,  (2020)

publication_link

 

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