ARG63038
Mouse anti-Bovine IgG (Light Chain) antibody [IVA285-1]
Mouse anti-Bovine IgG (Light Chain) 抗体 [IVA285-1] for Flow cytometry,IHC-Frozen sections,Western blot and Bovine
Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [IVA285-1] recognizes Bovine IgG (Light Chain) |
|---|---|
| 反应物种 | Bov |
| 应用 | FACS, IHC-Fr, WB |
| 特异性 | The clone IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | IVA285-1 |
| 同位型 | IgG1 |
| 靶点名称 | IgG (Light Chain) |
| 抗原 | Fraction of Bovine immunoglobulin. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | WB: Incubation: Overnight at 4°C. Sample preparation: Bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Under reducing condition. SDS-PAGE (12% separating gel). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: Bovine serum |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 研究领域 | Immune System antibody |
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检测图片 (1) Click the Picture to Zoom In
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ARG63038 Mouse anti-Bovine IgG (Light Chain) antibody [IVA285-1] WB image
Western blot: 1) Fetal calf serum, and 2) Bovine serum stained with ARG63038 Mouse anti-Bovine IgG (Light Chain) antibody [IVA285-1].
