ARG81310

Mouse/Rat HMGB1 ELISA Kit

Mouse/Rat HMGB1 ELISA 试剂盒 for ELISA and Mouse,Rat

publication_link 参考文献24

概述

产品描述 ARG81310 Mouse/Rat HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse/Rat HMGB1 in plasma, cell culture supernatants and cell/tissue lysate samples.
反应物种 Ms, Rat
应用 ELISA
特异性 Cross-react to mouse HMGB2 is less than 5%.
靶点名称 HMGB1
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm.

灵敏度 0.3 ng/ml
样品类型 Plasma, cell culture supernatants, Bronchoalveolar lavage fluid (BALF) and cell/tissue lysate samples.
标准范围 0.3125 - 20 ng/ml
样本量 100 µl
精确

< 5.2%

別名 HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

应用说明

检测时间 Overnight

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 15289 Mouse HMGB1

Swiss-port # P63158 Mouse High mobility group protein B1

基因名称 Hmgb1
全名 high mobility group box 1
背景介绍 HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能 HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
产品亮点 Related products:
HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
Total solution for HMGB1 research
HMGB1 in inflammation
Inflammatory Cytokines
HMGB1 ELISA Kit for your research
Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit
New HMGB1 neutralizing antibody is released
Detecting exosomal HMGB1 for ICD research
Related poster download:
HMGB1 Pathway.pdf
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex.

检测图片 (1) Click the Picture to Zoom In

  • ARG81310 Mouse/Rat HMGB1 ELISA Kit standard curve image

    ARG81310 Mouse/Rat HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

High Dose Local Photon Irradiation Is Crucial in Anti-CTLA-4 Antibody Therapy to Enhance the Abscopal Response in a Murine Pancreatic Carcinoma Model

ELISA / Mouse / Cell culture supernatants

Junya Yamamoto et al.
Cancers (Basel).,  (2022)

publication_link

 

hr_line

Assessment of the toxicity and carcinogenicity of double-walled carbon nanotubes in the rat lung after intratracheal instillation: a two-year study

ELISA / Rat / Pleural lavage fuid

Dina Mourad Saleh et al.
Part Fibre Toxicol,  (2022)

publication_link

 

hr_line

Distinct roles of HMGB1 in the regulation of P glycoprotein expression in the liver and kidney of mice with lipopolysaccharide-induced inflammation

ELISA / Mouse / Plasma; Tissue lysate

Atsushi Kawase et al.
Research Square,  (2022)

publication_link

 

hr_line

Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors

ELISA / Mouse / Serum , Cell culture supernatants

Fotis Nikolos et al.
Nat Commun.,  (2022)

publication_link

 

hr_line

Assessment of the Toxicity and Carcinogenicity of Double-Walled Carbon Nanotubes in the Rat Lung After Intratracheal Instillation: A Two-Year Study

ELISA / Rat / Pleural lavage fluid

Dina Mourad Saleh et al.
Research Square,  (2022)

publication_link

 

hr_line

Spatiotemporal depletion of tumor-associated immune checkpoint PD-L1 with near-infrared photoimmunotherapy promotes antitumor immunity

ELISA / Mouse / Cell culture supernatants

Shunichi Taki et al.
J Immunother Cancer.,  (2021)

publication_link

 

hr_line

Sodium Butyrate Alleviates Intestinal Inflammation in Mice with Necrotizing Enterocolitis

ELISA / Mouse / Tissue lysate

Qian Sun et al.
Mediators Inflamm.,  (2021)

publication_link

 

hr_line

Homologous-targeted and tumor microenvironment-activated hydroxyl radical nanogenerator for enhanced chemoimmunotherapy of non-small cell lung cancer

ELISA / Mouse Cell culture supernatant

LingxiaLiang et al.
Chemical Engineering Journal,  (2021)

publication_link

 

hr_line

Perfluorooctyl bromide nanoemulsions holding MnO2 nanoparticles with dual-modality imaging and glutathione depletion enhanced HIFU-eliciting tumor immunogenic cell death.

ELISA / Mouse / Cell culture medium

Xinping Kuai et al.
Acta Pharmaceutica Sinica B.,  (2021)

publication_link

 

hr_line

The Efficacy of Phage Therapy in a Murine Model of Pseudomonas aeruginosa Pneumonia and Sepsis.

ELISA / Mouse / Serum

Xu Yang et al.
Front. Microbiol.,  (2021)

publication_link

 

hr_line

High-density lipoprotein modulates tumor-associated macrophage for chemoimmunotherapy of hepatocellular carcinoma.

ELISA / Mouse / Cell culture medium

Junyang Wang et al.
Nano Today.,  (2021)

publication_link

 

hr_line

Glycyrrhizin ameliorates melanoma cell extravasation into mouse lungs by regulating signal transduction through HMGB1 and its receptors.

ELISA / Mouse / Plasma

Keiichi Hiramoto et al.
Journal of Clinical Biochemistry and Nutrition.,  (2021)

publication_link

 

hr_line

Clara cell 16 KDa protein mitigates house dust mite-induced airway inflammation and damage via regulating airway epithelial cell apoptosis in a manner dependent on HMGB1-mediated signaling inhibition.

ELISA / Mouse / BALF

Meixuan Liu et al.
Mol Med.,  (2021)

publication_link

 

hr_line

Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity.

ELISA / Mouse

Zhiwan Wang et al.
ACS Nano.,  (2021)

publication_link

 

hr_line

Combination of Cisplatin and Irradiation Induces Immunogenic Cell Death and Potentiates Postirradiation Anti-PD-1 Treatment Efficacy in Urothelial Carcinoma.

ELISA / Mouse / Cell culture supernatant

Hiroshi Fukushima et al.
Int J Mol Sci.,  (2021)

publication_link

 

hr_line

Comparison of Exosomes Derived from Non- and Gamma-Irradiated Melanoma Cancer Cells as a Potential Antigenic and Immunogenic Source for Dendritic Cell-Based Immunotherapeutic Vaccine.

ELISA / Mouse

Woo Sik Kim et al.
Vaccines (Basel).,  (2020)

publication_link

 

hr_line

Molecular hydrogen alleviates brain injury and cognitive impairment in a chronic sequelae model of murine polymicrobial sepsis.

ELISA / Mouse / Tissue lysate

Yi Jiang et al.
Exp Brain Res.,  (2020)

publication_link

 

hr_line

Tumor-derived HMGB1 induces CD62L dim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer.

ELISA / Mouse / Serum and culture medium

Zhen Wang et al.
Oncogenesis.,  (2020)

publication_link

 

hr_line

Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression.

ELISA / Mouse / Cell culture supernatant

Senbiao Chen et al.
Nano Today.,  (2020)

publication_link

 

hr_line

Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction.

ELISA / Rat

Liu Beilei et al.
Oxid Med Cell Longev.,  (2019)

publication_link

 

hr_line

Syringeable immunotherapeutic nanogel reshapes tumor microenvironment and prevents tumor metastasis and recurrence.

ELISA / Mouse

Song Chanyoung et al.
Nat Commun.,  (2019)

publication_link

 

hr_line

Targeting HMGB1 by ethyl pyruvate ameliorates systemic lupus erythematosus and reverses the senescent phenotype of bone marrow-mesenchymal stem cells.

ELISA / 

Ji Juan et al.
Aging.,  (2019)

publication_link

 

hr_line

Irradiation-induced osteocyte damage promotes HMGB1-mediated osteoclastogenesis in vitro.

ELISA / Mouse

He Feilong et al.
J Cell Physiol.,  (2019)

publication_link

 

hr_line

Carbon ion irradiation enhances the antitumor efficacy of dual immune checkpoint blockade therapy both for local and distant sites in murine osteosarcoma.

ELISA / Mouse

Takahashi Yutaka et al.
Oncotarget.,  (2019)

publication_link

 

hr_line
Title Download Link
ARG81310 Mouse/Rat HMGB1 ELISA Kit User's manual download icon Download