ARG81310

Mouse/Rat HMGB1 ELISA Kit

Mouse/Rat HMGB1 ELISA 试剂盒 for ELISA and Mouse,Rat

概述

产品描述

ARG81310 Mouse/Rat HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse HMGB1 in Mouse and Rat plasma, cell culture supernatants and cell/tissue lysate samples.

反应物种 Ms, Rat
应用 ELISA
特异性 Cross-react to mouse HMGB2 is less than 5%.
靶点名称 HMGB1
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm.

灵敏度 0.3 ng/ml
样品类型 Plasma, cell culture supernatants and cell/tissue lysate samples.
标准范围 0.3125 - 20 ng/ml
样本量 100 µl
精确

< 5.2%

全名 high mobility group box 1
別名 HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

应用说明

检测时间 Overnight

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 15289 Mouse HMGB1

Swiss-port # P63158 Mouse High mobility group protein B1

基因名称 Hmgb1
基因全名 high mobility group box 1
背景介绍 This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
生物功能 DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
产品亮点 Related products:
HMGB1 antibodies; HMGB1 ELISA Kits;
Related news:
Comprehensive HMGB1 ELISA Kits
Related poster download:
HMGB1 Pathway.pdf
Related blogs:
Stop spending time on looking for Mouse HMGB1 ELISA Kit! Go to arigo
Comprehensive HMGB1 ELISA Kits
翻译后修饰 Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex.

检测图片 (1) Click the Picture to Zoom In

  • ARG81310 Mouse/Rat HMGB1 ELISA Kit standard curve image

    ARG81310 Mouse/Rat HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81310 Mouse/Rat HMGB1 ELISA Kit User's manual download icon Download