Mouse OPN / Osteopontin ELISA Kit

Mouse OPN / Osteopontin ELISA 试剂盒 for ELISA and Mouse


货号 内含物名称 包装 温度
ARG81668-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81668-002 Standard 2 X 10 ng/vial 4°C
ARG81668-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81668-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81668-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81668-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81668-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81668-008 25X Wash buffer 20 ml 4°C
ARG81668-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81668-010 STOP solution 10 ml (Ready to use) 4°C
ARG81668-011 Plate sealer 4 strips Room temperature


产品描述 ARG81668 Mouse OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse OPN / Osteopontin in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
反应物种 Ms
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 OPN / Osteopontin
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 78 pg/ml
样品类型 Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
标准范围 156 - 10000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 6.1%; Inter-Assay CV: 7.0%
別名 BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1


检测时间 ~ 5 hours


形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 20750 Mouse SPP1

Swiss-port # P10923 Mouse Osteopontin

基因名称 SPP1
全名 secreted phosphoprotein 1
背景介绍 The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
生物功能 Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt]
产品亮点 Related products:
OPN antibodies; OPN ELISA Kits;
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翻译后修饰 Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.

N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG81668 Mouse OPN / Osteopontin ELISA Kit standard curve image

    ARG81668 Mouse OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81668 Mouse Osteopontin ELISA Kit User manual download icon Download