ARG81668
Mouse OPN / Osteopontin ELISA Kit
Mouse OPN / Osteopontin ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81668-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81668-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81668-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81668-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81668-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81668-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81668-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81668-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81668-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81668-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81668-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81668 Mouse OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse OPN / Osteopontin in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | OPN / Osteopontin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.0% |
| 別名 | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPP1 |
| 全名 | secreted phosphoprotein 1 |
| 背景介绍 | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 生物功能 | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| 产品亮点 | Related products: OPN antibodies; OPN ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81668 Mouse OPN / Osteopontin ELISA Kit standard curve image
ARG81668 Mouse OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81668 Mouse Osteopontin ELISA Kit User manual |
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