ARG70152

Mouse IL1 beta recombinant protein (Active) (His-tagged, C-ter)

Mouse IL1 beta recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

概述

产品描述 E. coli expressed, His-tagged (C-ter) Active Mouse IL1 beta recombinant protein
应用 SDS-PAGE
靶点名称 IL1 beta
物种 Mouse
A.A. 序列 MVPIR QLHYR LRDEQ QKSLV LSDPY ELKAL HLNGQ NINQQ VIFSM SFVQG EPSND KIPVA LGLKG KNLYL SCVMK DGTPT LQLES VDPKQ YPKKK MEKRF VFNKI EVKSK VEFES AEFPN WYIST SQAEH KPVFL GNNSG QDIID FTMES VSS with polyhistidine tag at the C-terminus.
表达系统 E. coli
活动 Active
活动说明 Determined by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 8 pg/ml. The specific activity of recombinant mouse IL-1 beta is approximately > 1.2x 10^8 IU/mg.
別名 Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

属性

形式 Powder
纯化 Ni-NTA chromatography.
纯化说明 Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度 > 98% (by SDS-PAGE)
缓冲液 PBS (pH 7.4)
重建 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放说明 Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 IL1B
全名 interleukin 1, beta
背景介绍 The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
生物功能 Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
细胞定位 Cytoplasm, cytosol. Lysosome. Secreted, exosome. Secreted. Note=The precursor is cytosolic. [UniProt]
产品亮点 Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins;
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Inflammatory Cytokines
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翻译后修饰 Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt]

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  • ARG70152 Mouse IL1 beta recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70152 Mouse IL1 beta recombinant protein (Active) (His-tagged, C-ter).