ARG82732
Human TPP1 ELISA Kit
Human TPP1 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82732-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82732-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82732-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82732-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82732-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82732-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82732-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82732-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82732-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82732-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82732-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82732 Human TPP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TPP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | TPP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.6% Inter-Assay CV: 6.0% |
| 別名 | EC 3.4.14.9; TPP-1; Tripeptidyl-peptidase I; GIG1; Lysosomal pepstatin-insensitive protease; SCAR7; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase 1; CLN2; LPIC; Cell growth-inhibiting gene 1 protein; TPP-I |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPP1 |
| 全名 | tripeptidyl peptidase I |
| 背景介绍 | This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:11054422, PubMed:19038966, PubMed:19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed:11054422, PubMed:19038966, PubMed:19038967). Requires substrates with an unsubstituted N-terminus (PubMed:19038966). [UniProt] |
| 细胞定位 | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| 产品亮点 | Related products: TPP1 antibodies; TPP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82732 Human TPP1 ELISA Kit standard curve image
ARG82732 Human TPP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82732 Human TPP1 ELISA kit User manual |
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