ARG81493
Human Progranulin ELISA Kit
Human Progranulin ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81493-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81493-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81493-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81493-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81493-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81493-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81493-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81493-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81493-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81493-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81493-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81493 Human Progranulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Progranulin in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Progranulin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 146 ng/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.8% Inter-Assay CV: 7.7% |
| 別名 | PEPI; Glycoprotein 88; CLN11; GEP; Proepithelin; Granulin E; Granulin D; Granulins; PGRN; Glycoprotein of 88 Kda; Granulin G; Progranulin; PCDGF; Granulin B; Granulin C; Granulin A; GP88; Granulin F |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GRN |
| 全名 | granulin |
| 背景介绍 | Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. [UniProt] |
| 产品亮点 | Related products: Progranulin antibodies; Progranulin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Granulins are disulfide bridged. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG81493 Human Progranulin ELISA Kit standard curve image
ARG81493 Human Progranulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81493 Human Progranulin ELISA Kit User's manual |
 Download
|
