ARG81268

Human OPN / Osteopontin ELISA Kit

Human OPN / Osteopontin ELISA 试剂盒 for ELISA and Human

publication_link 参考文献1

概述

产品描述 ARG81268 Human OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human OPN / Osteopontin in serum, plasma, urine or cell culture supernatants.
反应物种 Hu
应用 ELISA
靶点名称 OPN / Osteopontin
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm.

灵敏度 32 pg/ml
样品类型 Serum, plasma, urine or cell culture supernatants.
标准范围 62.5 - 4000 pg/ml
样本量 100 µl
別名 BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1

应用说明

检测时间 ~ 3.5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6696 Human SPP1

Swiss-port # P10451 Human Osteopontin

基因名称 SPP1
全名 secreted phosphoprotein 1
背景介绍 The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
生物功能 Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt]
产品亮点 Related products:
OPN antibodies; OPN ELISA Kits;
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翻译后修饰 Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.
N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

检测图片 (1) Click the Picture to Zoom In

  • ARG81268 Human OPN / Osteopontin ELISA Kit standard curve image

    ARG81268 Human OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

Transcriptomic analysis identifies upregulation of secreted phosphoprotein 1 in silicotic rats

ELISA / Human

Wenchen Cai et al.
Exp Ther Med.,  (2021)

publication_link

 

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Title Download Link
ARG81268 Human OPN / Osteopontin ELISA Kit User's manual download icon Download