ARG80101

Human IL1 beta ELISA Kit

Human IL1 beta ELISA 试剂盒 for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
publication_link 参考文献7

内含物

货号 内含物名称 包装 温度
ARG80101-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80101-002 Standard (Lyophilized) 3 X 0.6 ng/vial 4°C
ARG80101-003 Standard diluent buffer 20 ml 4°C
ARG80101-004 Antibody conjugate concentrate 400 µl 4°C
ARG80101-005 Antibody diluent buffer 16 ml 4°C
ARG80101-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80101-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80101-008 20X Wash buffer 50 ml 4°C
ARG80101-009 TMB substrate 12ml 4°C (Protect from light)
ARG80101-010 STOP solution 12ml 4°C
ARG80101-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG80101 Human IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 beta in Serum, Plasma, Cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 No significant cross-reactivity or interference with Human IL-1 alpha;mouse IL-1 alpha, IL-1 beta;rat IL-1 alpha;porcine IL-1 alpha, IL-1 beta
靶点名称 IL1 beta
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm

灵敏度 4 pg/ml
样品类型 Serum, Plasma, Cell culture supernatants
标准范围 7.8 - 500 pg/ml
样本量 100 µl
精确

CV: <10%

別名 Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

应用说明

检测时间 4 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

基因名称 IL1B
全名 interleukin 1, beta
背景介绍 IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
生物功能 IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
产品亮点 Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins;
Related news:
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New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
研究领域 Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
翻译后修饰 Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

检测图片 (1) Click the Picture to Zoom In

  • ARG80101 Human IL1 beta ELISA Kit standard curve image

    ARG80101 Human IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis

ELISA / Human / Culture supernatant

Wei-Ching Huang et al.
Respir Res.,  (2023)

publication_link

 

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IL-17C neutralization protects the kidney against acute injury and chronic injury

ELISA / Human / Cell culture supernatants

Fangfei Zhang et al.
EBioMedicine.,  (2023)

publication_link

 

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Macrophage secretory IL-1β promotes docetaxel resistance in head and neck squamous carcinoma via SOD2/CAT-ICAM1 signaling

ELISA / Human / Cell culture supernatant

Ching-Yun Hsieh et al.
JCI Insight,  (2022)

publication_link

 

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Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps.

ELISA / Human / Cell culture supernatant

Yan Wang et al.
Mol Med Rep.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ELISA / Human / Cell culture medium

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

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Role of Different Salmonella enterica Serotypes in THP-1 Macrophage-Derived Foam Cell Autophagy and Inflammatory Response.

ELISA / Human

Wong Min-Yi et al.
Sains Malaysiana.,  (2019)

publication_link

 

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Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis.

ELISA / Human

Jiang Jianfa et al.
Gynecol Endocrinol.,  (2019)

publication_link

 

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Title Download Link
ARG80101 Human IL1 beta ELISA Kit User manual download icon Download