ARG80171

Human Granzyme B ELISA Kit

Human Granzyme B ELISA 试剂盒 for ELISA and Human

Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit
publication_link 参考文献3

内含物

货号 内含物名称 包装 温度
ARG80171-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80171-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80171-003 Standard diluent buffer 20 ml 4°C
ARG80171-004 Antibody conjugate concentrate 400 µl 4°C
ARG80171-005 Antibody diluent buffer 16 ml 4°C
ARG80171-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80171-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80171-008 20X Wash buffer 50 ml 4°C
ARG80171-009 TMB substrate 12ml 4°C (Protect from light)
ARG80171-010 STOP solution 12ml 4°C
ARG80171-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG80171 Human Granzyme B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granzyme B in serum, plasma and cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 No significant cross-reactivity or interference with other soluble cytokine
靶点名称 Granzyme B
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm

灵敏度 8 pg/ml
样品类型 Serum, plasma and cell culture supernatants.
标准范围 15.6 - 1000 pg/ml
样本量 100 µl
精确

CV: <10%

別名 EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease

应用说明

检测时间 4 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3002 Human GZMB

Swiss-port # P10144 Human Granzyme B

基因名称 GZMB
全名 granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
背景介绍 Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
生物功能 Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]
产品亮点 Related products:
Granzyme B antibodies; Granzyme B ELISA Kits;
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研究领域 Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit

检测图片 (1) Click the Picture to Zoom In

  • ARG80171 Human Granzyme B ELISA Kit standard curve image

    ARG80171 Human Granzyme B ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.

ELISA / Human / Cell culture supernatant

Lu Huimin et al.
OncoImmunology.,  (2020)

publication_link

 

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Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.

ELISA / Human

Li Xiaomi et al.
Exp Cell Res.,  (2019)

publication_link

 

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The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.

ELISA / Human

Iketani Ryo et al.
Japanese Journal of Clinical Pharmacology and Therapeutics.,  (2019)

publication_link

 

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Title Download Link
ARG80171 Human Granzyme B ELISA Kit User's manual download icon Download