ARG81357

Dengue virus NS1 ELISA Kit

Dengue virus NS1 ELISA 试剂盒 for ELISA and Dengue virus

Microbiology and Infectious Disease antibody
publication_link 参考文献1

内含物

货号 内含物名称 包装 温度
ARG81357-001 antibody-coated microplate 12 X 8 strips 4°C
ARG81357-002 Standard (depends, e.g. 200 ng) 1 vial 4°C, store at -20 °C after reconstitution
ARG81357-003 Standard reconstitution buffer 1.1 mL 4°C
ARG81357-004 HRP-antibody Conjugate (200X) 115 μL 4°C
ARG81357-005 Assay buffer 12 mL 4°C
ARG81357-006 Detection antibody diluent buffer 12 mL 4°C
ARG81357-007 10X Wash buffer 20 mL 4°C
ARG81357-008 TMB substrate 12 mL (ready-to-use) 4°C (Protect from light)
ARG81357-009 STOP solution 6 mL 4°C
ARG81357-010 Plate sealer 1 strips Room temperature

概述

产品描述 ARG81357 Dengue virus NS1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Dengue virus NS1 in serum, plasma and cell culture supernatants.
反应物种 DEN
应用 ELISA
特异性 The ELISA kit reacts to DENV type 1-4 NS1 protein and it does not cross-react to ZIKV NS1 protein.
靶点名称 Dengue virus NS1
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 ~ 0.5 ng/ml
样品类型 Serum, plasma and cell culture supernatants.
标准范围 1.56 - 100 ng/ml
样本量 100 µl
全名 Dengue virus nonstructural protein 1
別名 Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody

应用说明

检测时间 ~ 2.5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 DENV_gp1
基因全名 Dengue virus nonstructural protein 1
背景介绍 Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic.
生物功能 Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity). [Uniprot]
产品亮点 Related products:
Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels;
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研究领域 Microbiology and Infectious Disease antibody

检测图片 (1) Click the Picture to Zoom In

  • ARG81357 Dengue virus NS1 ELISA Kit standard curve image

    ARG81357 Dengue virus NS1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

High Levels of Serum Hyaluronan Predicts Severe Dengue Infection and Perturbs Vascular Integrity.

ELISA / Dengue virus

Lin Chun-Yu et al.
Preprint,  (2019)

publication_link

 

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Title Download Link
ARG81357 Dengue virus NS1 ELISA Kit User's manual download icon Download