ARG83729
arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit
arigoQIK® Mouse CD274 / PD-L1 ELISA Development 试剂盒 for ELISA and Mouse
概述
| 产品描述 | ARG83729 arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution. This ELISA Development Kit is designed for the development of sandwich ELISA to measure Mouse CD274 / PD-L1 in Serum, plasma and cell culture supernatants. For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit) More aboutarigoQIK® : ● Optimized capture and detection antibody pairs ● Reduced incubation time and wash cycles ● 2-hour quicker than conventional ELISA process ● 5- and 15-plate packages available |
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| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CD274 / PD-L1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 4 pg/mL |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 7.81-500 pg/mL |
| 样本量 | 50 μL |
| 精确 | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| 別名 | CD274; CD274 Molecule; PD-L1; B7H1; PDL1; Programmed Cell Death 1 Ligand 1; CD274 Antigen; B7 Homolog 1; PDCD1LG1; B7-H1; B7-H; PDCD1 Ligand 1; PDCD1L1; HPD-L1; Programmed Death Ligand 1 |
属性
| 存放说明 | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
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| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | CD274 |
| 全名 | CD274 Molecule |
| 背景介绍 | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [Uniprot] |
| Title | Download Link |
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| ARG83729 arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit User manual |
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