ARG66645

anti-vWF antibody [SQab19164]

anti-vWF antibody [SQab19164] for IHC-Formalin-fixed paraffin-embedded sections and Human

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概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab19164] recognizes vWF
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab19164
同位型 IgG
靶点名称 vWF
抗原物种 Human
抗原 Synthetic peptide within aa. 1150-1250 of Human von Willebrand factor (vWF).
偶联标记 Un-conjugated
別名 VWD; von Willebrand factor; vWF; von Willebrand antigen II; F8VWF

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 7450 Human VWF

Swiss-port # P04275 Human von Willebrand factor

基因名称 VWF
全名 von Willebrand factor
背景介绍 The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
生物功能 Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. [UniProt]
细胞定位 Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. [UniProt]
预测分子量 309 kDa
翻译后修饰 All cysteine residues are involved in intrachain or interchain disulfide bonds.

N- and O-glycosylated. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66645 anti-vWF antibody [SQab19164] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human cervix tissue stained with ARG66645 anti-vWF antibody [SQab19164]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).

客户反馈

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anti-vWF antibody [SQab19164]

Application:IHC

Sample:Mouse Blood vessel

Sample Preparation Method:Paraffin

Fixation Buffer:4% PFA

Fixation Time:Overnight

Fixation Temperature:4 ºC

Antigen Retrieval Buffer:EDTA

Antigen Retrieval Time:20 min

Antigen Retrieval Temperature:95 ºC ºC

Primary Antibody Dilution Factor:1 : 100

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC