ARG42214
anti-vWF antibody [3E2D10]
anti-vWF antibody [3E2D10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [3E2D10] recognizes vWF |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3E2D10 |
| 同位型 | IgG1, kappa |
| 靶点名称 | vWF |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 845-949 of Human vWF. |
| 偶联标记 | Un-conjugated |
| 別名 | VWD; von Willebrand factor; vWF; von Willebrand antigen II; F8VWF |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | VWF |
| 全名 | von Willebrand factor |
| 背景介绍 | This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015] |
| 生物功能 | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. [UniProt] |
| 细胞定位 | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. [UniProt] |
| 预测分子量 | 309 kDa |
| 翻译后修饰 | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG42214 anti-vWF antibody [3E2D10] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. The tissue section was stained with ARG42214 anti-vWF antibody [3E2D10].
-
ARG42214 anti-vWF antibody [3E2D10] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human pancreas tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. The tissue section was stained with ARG42214 anti-vWF antibody [3E2D10].
