概述

产品描述	Rabbit Polyclonal antibody recognizes tPA
反应物种	Hu, Ms, Rat
应用	IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	tPA
抗原物种	Human
抗原	Synthetic peptide derived from Human tPA.
偶联标记	Un-conjugated
別名	TPA; tPA; T-PA; t-plasminogen activator; t-PA; Tissue-type plasminogen activator; EC 3.4.21.68; Reteplase; Alteplase

应用说明

应用建议	应用 推荐稀释比 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 18791 Mouse PLAT GeneID: 25692 Rat PLAT GeneID: 5327 Human PLAT Swiss-port # P00750 Human Tissue-type plasminogen activator Swiss-port # P11214 Mouse Tissue-type plasminogen activator Swiss-port # P19637 Rat Tissue-type plasminogen activator More
基因名称	PLAT
全名	plasminogen activator, tissue
背景介绍	This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
生物功能	Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. [UniProt]
细胞定位	Secreted, extracellular space. [UniProt]
预测分子量	63 kDa
翻译后修饰	The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line. [UniProt]

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