ARG54620
anti-p53R2 antibody
anti-p53R2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes p53R2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | This antibody recognizes an epitope atthe N-terminus of human, mouse, andrat p53R2, a p53 inducible gene thatcontains a p53 binding sequence andencodes a subunit of the enzymeribonucleotide reductase. p53R2 isinduced by agents that cause DNAdamage, such as UV and γ-irradiation.The product of the p53R2 gene isdirectly involved in the p53 checkpointfor repair of damaged DNA. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | p53R2 |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa2-17 of human p53R2 |
| 偶联标记 | Un-conjugated |
| 別名 | P53R2; EC 1.17.4.1; p53-inducible ribonucleotide reductase small subunit 2-like protein; TP53-inducible ribonucleotide reductase M2 B; Ribonucleoside-diphosphate reductase subunit M2 B; MTDPS8A; MTDPS8B; p53R2 |
应用说明
| 应用建议 |
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| 应用说明 | Western blot: use at 0.5 - 1 ug/ml. In immunoblots, a band of 39 kD is detected * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6PEE3 Mouse Ribonucleoside-diphosphate reductase subunit M2 B Swiss-port # Q7LG56 Human Ribonucleoside-diphosphate reductase subunit M2 B |
|---|---|
| 基因名称 | RRM2B |
| 全名 | ribonucleotide reductase M2 B (TP53 inducible) |
| 背景介绍 | This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010] |
| 生物功能 | Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | 41 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG54620 anti-p53R2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung stained with ARG54620 anti-p53R2 antibody at 1 µg/ml dilution.
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ARG54620 anti-p53R2 antibody WB image
Western blot: A431 cell lysate stained with ARG54620 anti-p53R2 antibody at 1 µg/ml dilution.
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ARG54620 anti-p53R2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung stained with ARG54620 anti-p53R2 antibody at 20 μg/ml dilution.
