ARG51850

anti-p38 MAPK phospho (Thr180 / Tyr182) antibody

anti-p38 MAPK phospho (Thr180 / Tyr182) antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody
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publication_link 参考文献8

概述

产品描述 Rabbit Polyclonal antibody recognizes p38 MAPK phospho (Thr180 / Tyr182)
反应物种 Hu, Ms, Rat
预测物种 Cow
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 p38 MAPK
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of threonine 180 or tyrosine 182 (E-M-T(p)-G-Y(p)-V-A) derived from Human P38 MAPK.
偶联标记 Un-conjugated
別名 CSBP2; PRKM14; MAX-interacting protein 2; SAPK2A; Stress-activated protein kinase 2a; CSBP1; Mxi2; EC 2.7.11.24; CSBP; SAPK2a; RK; CSPB1; MAPK 14; p38; MAP kinase 14; MAP kinase MXI2; Cytokine suppressive anti-inflammatory drug-binding protein; MAP kinase p38 alpha; Mitogen-activated protein kinase p38 alpha; EXIP; CSAID-binding protein; p38ALPHA; PRKM15; Mitogen-activated protein kinase 14

应用说明

预测反应性说明 Cow (100%)
应用建议
应用 推荐稀释比
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1432 Human MAPK14

GeneID: 26416 Mouse MAPK14

GeneID: 81649 Rat MAPK14

基因名称 MAPK14
全名 mitogen-activated protein kinase 14
背景介绍 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能 Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover.
产品亮点 Related Antibody Duos and Panels:
ARG30205 NFkB Activation Antibody Panel
ARG30272 Phospho p38 MAPK Antibody Duo (Total, pThr180/Tyr182)
Related products:
P38 MAPK antibodies; P38 MAPK Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域 Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody
预测分子量 41 kDa
翻译后修饰 Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 (PubMed:21283629).
Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.
Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.

检测图片 (1) Click the Picture to Zoom In

  • ARG51850 anti-p38 MAPK phospho (Thr180 / Tyr182) antibody WB image

    Western blot: 30 µg of HeLa treated with TNFa at 0, 10, 20, and 30 min. The blots stained with ARG51850 anti-p38 MAPK phospho (Thr180 / Tyr182) antibody, ARG55258 anti-p38 MAPK antibody, and ARG65714 anti-Vinculin antibody.

客户反馈

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anti-p38 MAPK phospho (Thr180 / Tyr182) antibody

Application:WB

Sample:HeLa treated with TNFa at 0, 10, 20, and 30 min

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Structural characterization and immunomodulatory activity of an acidic polysaccharide from walnut green husk

WB / Mouse

Guoliang Wang et al.
Journal of Functional Foods,  (2023)

publication_link

 

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Integrin CD11b Contributes to Hypertension and Vascular Dysfunction Through Mediating Macrophage Adhesion and Migration

WB / Mouse

Qiu-Yue Lin et al.
Hypertension.,  (2023)

publication_link

 

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DHPA Protects SH-SY5Y Cells from Oxidative Stress-Induced Apoptosis via Mitochondria Apoptosis and the Keap1/Nrf2/HO-1 Signaling Pathway

WB / Human

Yunhui Cai  et al.
Antioxidants (Basel),  (2022)

publication_link

 

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Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

WB / Human

Peng-Hsiang Fang et al.
Molecular Medicine,  (2022)

publication_link

 

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Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms

WB / Mouse

Jie Bai et al.
Oxid Med Cell Longev.,  (2022)

publication_link

 

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Shikonin Induced Program Cell Death through Generation of Reactive Oxygen Species in Renal Cancer Cells

WB / Human

Ming-Feng Tsai et al.
Antioxidants (Basel).,  (2021)

publication_link

 

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Angiotensin-(1-7) treatment blocks lipopolysaccharide-induced organ damage, platelet dysfunction, and IL-6 and nitric oxide production in rats.

WB / Rat

Hsin-Jung Tsai et al.
Sci Rep.,  (2021)

publication_link

 

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The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

WB / Human

Jingchun Du et al.
Stem Cells Int.,  (2019)

publication_link

 

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