ARG58845
anti-hnRNP K antibody
anti-hnRNP K antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes hnRNP K |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | hnRNP K |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-280 of Human hnRNP K (NP_112552.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Heterogeneous nuclear ribonucleoprotein K; TUNP; HNRPK; CSBP; hnRNP K; Transformation up-regulated nuclear protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse testis | ||||||||
| 实际分子量 | 67 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HNRNPK |
| 全名 | heterogeneous nuclear ribonucleoprotein K |
| 背景介绍 | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. [UniProt] |
| 细胞定位 | Cell projection, Cytoplasm, Nucleus, nucleoplasm, podosome. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions. Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58845 anti-hnRNP K antibody ICC/IF image
Immunofluorescence: MCF-7 cells stained with ARG58845 anti-hnRNP K antibody.
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ARG58845 anti-hnRNP K antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung stained with ARG58845 anti-hnRNP K antibody at 1:100 dilution.
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ARG58845 anti-hnRNP K antibody WB image
Western blot: 25 µg of Mouse testis lysate stained with ARG58845 anti-hnRNP K antibody at 1:1000 dilution.
