ARG58784
anti-hemoglobin beta + delta antibody
anti-hemoglobin beta + delta antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
产品描述 | Rabbit Polyclonal antibody recognizes hemoglobin beta + delta |
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反应物种 | Hu, Ms, Rat |
应用 | IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | hemoglobin beta + delta |
抗原物种 | Human |
抗原 | Recombinant protein corresponding to V2-H147 of Human hemoglobin delta. |
偶联标记 | Un-conjugated |
別名 | Delta-globin; Hemoglobin delta chain; Hemoglobin subunit delta |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | HBD |
全名 | hemoglobin, delta |
背景介绍 | The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia. [provided by RefSeq, Jul 2008] |
生物功能 | Involved in oxygen transport from the lung to the various peripheral tissues. [UniProt] |
预测分子量 | 16 kDa |
检测图片 (8) Click the Picture to Zoom In
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta, Rat spleen, Mouse spleen lysates stained with ARG58784 anti-hemoglobin beta + delta antibody at 0.5 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta lysate stained with ARG58784 anti-hemoglobin beta + delta antibody at 0.5 µg/ml, overnight at 4°C.