ARG58657
anti-eRF1 antibody
anti-eRF1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eRF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eRF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 278-437 of Human eRF1 (NP_004721.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Protein Cl1; RF1; eRF1; ERF1; Eukaryotic release factor 1; Eukaryotic peptide chain release factor subunit 1; ERF; TB3-1; SUP45L1; D5S1995 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HT1080 | ||||||
| 实际分子量 | 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ETF1 |
| 全名 | eukaryotic translation termination factor 1 |
| 背景介绍 | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013] |
| 生物功能 | Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 49 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG58657 anti-eRF1 antibody WB image
Western blot: 25 µg of HT1080 cell lysate stained with ARG58657 anti-eRF1 antibody at 1:1000 dilution.
