ARG58612
anti-eRF1 antibody
anti-eRF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eRF1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Gpig, Hrs, Rb, Yeast, Zfsh |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eRF1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminal region of Human eRF1. (within the following sequence: ISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKL) |
| 偶联标记 | Un-conjugated |
| 別名 | Protein Cl1; RF1; eRF1; ERF1; Eukaryotic release factor 1; Eukaryotic peptide chain release factor subunit 1; ERF; TB3-1; SUP45L1; D5S1995 |
应用说明
| 预测反应性说明 | Predicted homology based on immunogen sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 93%; Zebrafish: 100% | ||||||
|---|---|---|---|---|---|---|---|
| 应用建议 |
|
||||||
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | THP-1 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 浓度 | Batch dependent: 0.5 - 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P62495 Human Eukaryotic peptide chain release factor subunit 1 |
|---|---|
| 基因名称 | ETF1 |
| 全名 | eukaryotic translation termination factor 1 |
| 背景介绍 | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013] |
| 生物功能 | Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. [UniProt] |
| 预测分子量 | 49 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG58612 anti-eRF1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver stained with ARG58612 anti-eRF1 antibody (orange-red) at 1:100 dilution.
-
ARG58612 anti-eRF1 antibody WB image
Western blot: THP-1 cell lysate stained with ARG58612 anti-eRF1 antibody at 0.2 - 1 µg/ml dilution.
